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Improvement in the viability of cryopreserved cells by microencapsulation.

Author(s) : MATSUMOTO Y., MORINAGA Y., UJIHIRA M., et al.

Type of article: Article

Summary

The advantages of microencapsulated cells over those of suspended cells were evaluated for improving viability in cryopreservation. Rat pheochromocytoma (PC12) cells were selected as the test biological cells and microencapsulated PC12 in alginate-polylysine-alginate membranes. These microencapsulated PC12 cells were frozen by differential scanning calorimetry (DSC) at various cooling rates of 0.5 to 10 °C/min. Their latent heat was measured during freezing from 4 to -80 °C. The post-thaw viability was evaluated. Results showed that at cooling rates of 0.5 and 1 °C/min, the latent heat of microencapsulated PC12 cells was lower than that of suspended cells. The post-thaw viability of microencapsulated PC12 cells was improved when the cooling rate was 0.5 or 1 °C/min, compared with that of suspended cells.

Details

  • Original title: Improvement in the viability of cryopreserved cells by microencapsulation.
  • Record ID : 2003-1599
  • Languages: English
  • Source: JSME int. J., C - vol. 44 - n. 4
  • Publication date: 2001/12
  • Document available for consultation in the library of the IIR headquarters only.

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