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Ensilication improves the thermal stability of the tuberculosis antigen Ag85b and an Sbi-Ag85b vaccine conjugate.

L'encapsulation dans une matrice de silice améliore la stabilité thermique de l'antigène de la tuberculose Ag85b et d'un vaccin conjugué Sbi-Ag85b.

Auteurs : WAHID A. A., SARTBAEVA A., VAN DEN ELSEN J. M. H.

Type d'article : Article

Résumé

There is an urgent need for the development of vaccine thermostabilisation methodologies as the maintenance of a continuous and reliable cold chain remains a major hurdle to the global distribution of safe and effective vaccines. Ensilication, a method that encases proteins in a resistant silica cage has been shown to physically prevent the thermal denaturation of a number of model proteins. In this study we investigate the utility of this promising approach in improving the thermal stability of antigens and vaccine conjugates highly relevant to the development of candidate tuberculosis vaccines, including antigen 85b conjugated with the Staphylococcus aureus-protein based adjuvant Sbi. Here we analyse the sensitivity of these constructs to thermal denaturation and demonstrate for the first time the benefits of ensilication in conferring these vaccine-relevant proteins with protection against temperature-induced loss of structure and function without the need for refrigeration. Our results reveal the potential of ensilication in facilitating the storage and transport of vaccines at ambient temperatures in the future and therefore in delivering life-saving vaccines globally, and in particular to remote areas of developing countries where disease rates are often highest.

Détails

  • Titre original : Ensilication improves the thermal stability of the tuberculosis antigen Ag85b and an Sbi-Ag85b vaccine conjugate.
  • Identifiant de la fiche : 30027075
  • Langues : Anglais
  • Sujet : Pays en développement
  • Source : Nature Scientific Reports - vol. 9
  • Date d'édition : 08/08/2019
  • DOI : http://dx.doi.org/doi.org/10.1038/s41598-019-47657-9

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