• Accueil
  • Publications

  • Using liquid nitrogen for conifer plant seed co...

Document IIF

Using liquid nitrogen for conifer plant seed conservation.

Utilisation d’azote liquide pour la conservation des graines de conifères.

Numéro : pap. n. 02

Auteurs : ARAPETIAN E., USATENKO Y., SCHERBINA M.

Résumé

Ex situ preservation of seeds has been studied using a cryotechnique. Germplasm was in the form of seeds of the species genus Abies Mill., Cryptomeria Don, Metasequoia Miki, Pinus L., Platycladus Spach, Sequoiadendron Buchholz, Taxus L. and Tsuga Carr. The seeds were collected from the introduced conifer trees which are grown in the collection of the Lviv Botanical garden. The seeds packed in tight paper tubes without preliminary chemical treatment were dipped in liquid nitrogen (LN -196 °C) using direct immersion for a period of one month. After seed stratification, their soil germination in laboratory conditions was the same for a seed control variant (storage under 25-27 °C) and experiment (storage in LN). The treatment of the seeds with a chemical germination stimulus before planting did not affect their germinating ability. The experiments carried out over several years with the seeds of conifers of local introduction showed no influence of the physical or chemical exposure factors on the germination rate.

Documents disponibles

Format PDF

Pages : 4

Disponible

  • Prix public

    15 €

  • Prix membre*

    Gratuit

* meilleur tarif applicable selon le type d'adhésion (voir le détail des avantages des adhésions individuelles et collectives)

Détails

  • Titre original : Using liquid nitrogen for conifer plant seed conservation.
  • Identifiant de la fiche : 30021680
  • Langues : Anglais
  • Source : Cryogenics 2017. Proceedings of the 14th IIR International Conference: Dresden, Germany, Mai 15-19, 2017.
  • Date d'édition : 15/05/2017
  • DOI : http://dx.doi.org/10.18462/iir.cryo.2017.0002

Liens


Voir d'autres communications du même compte rendu (71)
Voir le compte rendu de la conférence