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Cryoconservation des cellules hépatiques d'homme adulte issues d'une résection du foie.

Cryopreservation of adult human hepatocytes obtained from resected liver biopsies.

Auteurs : ALEXANDRE E., VIOLLON-ABADIE C., DAVID P., et al.

Type d'article : Article

Résumé

Isolated human hepatocytes have been shown to represent a valuable in vitro model to investigate the metabolism and cytotoxicity of xenobiotics. In addition, human hepatocyte transplantation and artificial liver support systems using isolated human hepatocytes are currently investigated as treatment for acute and chronic hepatic failure. In this regard, human hepatocyte banking by cryopreservation would be of great interest. In the study, freshly isolated hepatocytes from resected liver biopsies of 28 separate donors were cryopreserved using two different protocols, stepwise freezing (SF) or progressive freezing (PF), in combination (PF(+), SF(+)) or not (PF(-), SF(-)) with a 30 min preincubation in culture medium at 37 °C. Total recovery was higher after PF than after SF. The results of the study suggest that recovery of human hepatocytes after isolation prior to progressive freezing should allow human hepatocyte banking for use in pharmacotoxicology and cell therapy research purposes.

Détails

  • Titre original : Cryopreservation of adult human hepatocytes obtained from resected liver biopsies.
  • Identifiant de la fiche : 2003-2046
  • Langues : Anglais
  • Source : Cryobiology - vol. 44 - n. 2
  • Date d'édition : 04/2002

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