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Caractérisation des cellules de Langerhans humaines cryoconservées.

Characterization of cryopreserved human Langerhans cells.

Auteurs : SEO K. I., HUH C. H., HAN J. H., et al.

Type d'article : Article

Résumé

Epidermal Langerhans cells are potent antigen-presenting cells in the epidermis. The establishment of a cryopreservation method for human Langerhans cells would greatly contribute to our ability to successfully conduct various experiments. Since Langerhans cells are known to be sensitive to cold injury, there have been no reports concerning the cryopreservation of Langerhans cells. The authors have investigated the effect of cryopreservation on the function and phenotype of human Langerhans cells. Langerhans cells were cryopreserved in the presence of dimethylsulfoxide (DMSO) 10% and fetal calf serum 90%. Cryopreserved Langerhans cells were phenotypically assessed by flow cytometry using monoclonal antibodies. The ultrastructures of the cells were compared using electron microscopy. An autologous T cell stimulation test was performed to compare the functions of cryopreserved Langerhans cells and fresh Langerhans cells. The viability of the cryopreserved Langerhans cells was able to be maintained at more than 90%. The authors' findings indicate that the cryopreservation of human Langerhans cells could lead to a breakthrough in various experiments dealing with these cells.

Détails

  • Titre original : Characterization of cryopreserved human Langerhans cells.
  • Identifiant de la fiche : 2003-3036
  • Langues : Anglais
  • Source : Cryobiology - vol. 45 - n. 2
  • Date d'édition : 10/2002

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