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Cryoconservation de l'îlot pancréatique de Langerhans chez le rat en fonction de la vitesse de refroidissement.

[In Japanese. / En japonais.]

Auteurs : MORIYA H., YAMAGUCHI S.

Type d'article : Article

Résumé

It has been well shown that rat pancreatic islet can be cryopreserved and continue to have the ability to secrete insulin under a wide range of cooling rates. On the other hand, lymphocytes can not tolerate the freezing and thawing process of rapid cooling in excess of the optimum cooling rate as a single cell suspension in the solution. Therefore there may be a possibility that rapid cooling can destroy the passenger leukocytes and cause immunomodulation of pancreatic islet. The problem is that when pancreatic islets preserved using rapid cooling exceeding the optimum cooling rate of both lymphocytes and pancreatic islets, the viability of pancreatic islets decreased and that could not ameliorate hyperglycemia. The slow cooling group showed almost the same glucose decay curve as freshly isolated control group and the K-value of IVGTT was in the normal range. Rapidly cooled islet decreased hyperglycemia but could not reach normal glucose level when the number of transplanted islets was insufficient, but when sufficient numbers of islets were transplanted animals achieved normoglycemia and showed normal K-values of IVGTT.

Détails

  • Titre original : [In Japanese. / En japonais.]
  • Identifiant de la fiche : 2004-2517
  • Langues : Japonais
  • Source : Low Temperature Medicine - vol. 25 - n. 3
  • Date d'édition : 09/1999
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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