Share

Cryopreservation of rat pancreatic islet of Langerhans using slow and fast cooling.

[In Japanese. / En japonais.]

Author(s) : MORIYA H., YAMAGUCHI S.

Type of article: Article

Summary

It has been well shown that rat pancreatic islet can be cryopreserved and continue to have the ability to secrete insulin under a wide range of cooling rates. On the other hand, lymphocytes can not tolerate the freezing and thawing process of rapid cooling in excess of the optimum cooling rate as a single cell suspension in the solution. Therefore there may be a possibility that rapid cooling can destroy the passenger leukocytes and cause immunomodulation of pancreatic islet. The problem is that when pancreatic islets preserved using rapid cooling exceeding the optimum cooling rate of both lymphocytes and pancreatic islets, the viability of pancreatic islets decreased and that could not ameliorate hyperglycemia. The slow cooling group showed almost the same glucose decay curve as freshly isolated control group and the K-value of IVGTT was in the normal range. Rapidly cooled islet decreased hyperglycemia but could not reach normal glucose level when the number of transplanted islets was insufficient, but when sufficient numbers of islets were transplanted animals achieved normoglycemia and showed normal K-values of IVGTT.

Details

  • Original title: [In Japanese. / En japonais.]
  • Record ID : 2004-2517
  • Languages: Japanese
  • Source: Low Temperature Medicine - vol. 25 - n. 3
  • Publication date: 1999/09
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (1)
See the source

Indexing