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Cryoconservation du tissu ovarien bovin : intégrité structurelle des follicules suite à la décongélation et la culture in vitro.

Cryopreservation of bovine ovarian tissue: structural normality of follicles after thawing and culture in vitro.

Auteurs : PAYNTER S. J., COOPER A., FULLER B. J., et al.

Type d'article : Article

Résumé

The recovery of viable follicles from cryopreserved ovarian tissue would be of benefit in many areas of assisted reproduction. Structural integrity needs to be maintained following cryopreservation of ovarian tissue in order to retrieve healthy follicles which can then be cultured in vitro to produce viable oocytes. The authors have assessed the effect of in vitro culture of bovine tissue for 0, 1, 4, 24, or 48 h after exposure to, or cryopreservation in, DMSO. The data obtained indicate that it is possible to freeze/thaw bovine ovarian tissue while retaining a reasonable yield of morphologically intact follicles and that a short period of post-thaw culture may enhance follicle recovery.

Détails

  • Titre original : Cryopreservation of bovine ovarian tissue: structural normality of follicles after thawing and culture in vitro.
  • Identifiant de la fiche : 2000-2226
  • Langues : Anglais
  • Source : Cryobiology - vol. 38 - n. 4
  • Date d'édition : 06/1999

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