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Du flacon à la plaque de culture multipuits : congélation à vitesse contrôlée dans un environnement convectif.

From vial to multiwell - Controlled rate freezing in a convective environment.

Numéro : pap. n. 14

Auteurs : REINSCH H., KRETSCHMER R., SPOERL G.

Résumé

Sophisticated controlled rate freezers for biological samples are operated by the gasification of liquid nitrogen. A controlled circulation of the cold nitrogen gas within the freezer chamber provides a high cooling performance as well as outstanding freezing rates. Thus, those computer-controlled chamber freezers should allow a reproducible success in tissue freezing. Unfortunately the inconsistent flow of the cold nitrogen gas causes distinct spatial differences of sample temperatures and freezing rates at different positions of the freezing chamber. For this reason, a thermal management unit has been successfully developed for the simultaneous freezing of three tissue transplants with a maximum size of 5 cm². While the underlying concept of passive temperature compensation was very successful for the cryopreservation of this small amount of specimen, it reaches its limits in multiwell freezing. Novel strategies have to be developed for the tissue cryopreservation in multiwell plates with LN2-operated convection freezers.

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Pages : 5

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Détails

  • Titre original : From vial to multiwell - Controlled rate freezing in a convective environment.
  • Identifiant de la fiche : 30021692
  • Langues : Anglais
  • Source : Cryogenics 2017. Proceedings of the 14th IIR International Conference: Dresden, Germany, Mai 15-19, 2017.
  • Date d'édition : 15/05/2017
  • DOI : http://dx.doi.org/10.18462/iir.cryo.2017.0014

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