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KrioBlast™, une plateforme pour la vitrification cinétique par refroidissement "click" (hyper rapide) : applications pour la cryoconservation et les banques cryogéniques.

KrioBlast™, a platform for kinetic vitrification by “click” (hyperfast) cooling: applications for cryopreservation and cryobanking.

Numéro : pap. n. 11

Auteurs : KATKOV I. I., BOLYUKH V. F., LIU Y., et al.

Résumé

Kinetic (very rapid) vitrification (K-VF) is a promising approach for cryopreservation (CP) of biological materials and cryobanking for reproductive and regenerative medicine, husbandry, and cryopreservation of wildlife genetic resources as it is simple, robust, and can achieve VF for practically any type of cells. Up to know, superfast K-VF methods require very small sample size and cannot avoid the Leidenfrost effect (LFE), which substantially impedes the rate of cooling. Here, we are reporting an entirely novel system “KrioBlast(TM)“ for hyper-fast K-VF (“click” cooling) of one-two order of magnitude larger samples. The system completely eliminates LFE and a need of potentially toxic and osmotically damaging vitrificants such as DMSO or ethylene glycol used in the current methods of VF. Preliminary experiments have shown feasibility and potentially very high survival of cryopreserved human spermatozoa and embryonic stem cells.

Documents disponibles

Format PDF

Pages : 6 p.

Disponible

  • Prix public

    20 €

  • Prix membre*

    Gratuit

* meilleur tarif applicable selon le type d'adhésion (voir le détail des avantages des adhésions individuelles et collectives)

Détails

  • Titre original : KrioBlast™, a platform for kinetic vitrification by “click” (hyperfast) cooling: applications for cryopreservation and cryobanking.
  • Identifiant de la fiche : 30011203
  • Langues : Anglais
  • Source : Cryogenics 2014. Proceedings of the 13th IIR International Conference: Prague, Czech Republic, April 7-11, 2014.
  • Date d'édition : 07/04/2014

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