Partager

Iinfluence d'un régime de refroidissement triétagé et de la nucléation de glace induite pendant la cryoconservation sur le potentiel de formation de colonies et la différentiation dans les cellules souches mésenchymales provenant du foie foetal humain.

Importance of three-stage cooling regime and induced ice nucleation during cryopreservation on colony-forming potential and differentiation in mesenchymal stem/progenitor cells from human fetal liver.

Auteurs : SKOROBOGATOVA N. G., NOVIKOV A. N., FULLER B. J., et al.

Type d'article : Article

Résumé

The colony-forming and osteogenic and adipogenic capacities of primary human fetal liver mesenchymal stem or progenitor cells were compared with respect to a 3-step cooling protocol and the same protocol with ice nucleation along with 5 and 10% Me2SO. It was established that a slow freezing protocol with 10% Me2SO and induced ice nucleation best protects the unexpanded primary suspension of human fetal liver mesenchymal stem cells and enables renewal during subsequent culture and directed differentiation to osteo- or adipogenic lineages.

Détails

  • Titre original : Importance of three-stage cooling regime and induced ice nucleation during cryopreservation on colony-forming potential and differentiation in mesenchymal stem/progenitor cells from human fetal liver.
  • Identifiant de la fiche : 30003537
  • Langues : Anglais
  • Source : CryoLetters - vol. 31 - n. 5
  • Date d'édition : 09/2010

Liens


Voir d'autres articles du même numéro (2)
Voir la source

Indexation