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Importance of three-stage cooling regime and induced ice nucleation during cryopreservation on colony-forming potential and differentiation in mesenchymal stem/progenitor cells from human fetal liver.

Author(s) : SKOROBOGATOVA N. G., NOVIKOV A. N., FULLER B. J., et al.

Type of article: Article

Summary

The colony-forming and osteogenic and adipogenic capacities of primary human fetal liver mesenchymal stem or progenitor cells were compared with respect to a 3-step cooling protocol and the same protocol with ice nucleation along with 5 and 10% Me2SO. It was established that a slow freezing protocol with 10% Me2SO and induced ice nucleation best protects the unexpanded primary suspension of human fetal liver mesenchymal stem cells and enables renewal during subsequent culture and directed differentiation to osteo- or adipogenic lineages.

Details

  • Original title: Importance of three-stage cooling regime and induced ice nucleation during cryopreservation on colony-forming potential and differentiation in mesenchymal stem/progenitor cells from human fetal liver.
  • Record ID : 30003537
  • Languages: English
  • Source: CryoLetters - vol. 31 - n. 5
  • Publication date: 2010/09

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