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Incorporation du diméthylsulfoxyde (DMSO) et du dextran 40 dans un hydrogel à base de gélatine et d'alginate pour la cryoconservation contrôlée des cellules assemblées.

Incorporation of DMSO and dextran-40 into a gelatin/alginate hydrogel for controlled assembled cell cryopreservation.

Auteurs : WANG X., XU H.

Type d'article : Article

Résumé

A new cell cryopreservation strategy for cell-assembling constructs was proposed. With this strategy, different concentrations of dimethysulfoxide (DMSO) and dextran-40 were directly incorporated into the cell/gelatin/alginate systems, prototyped according to a predesigned structure, cryopreserved at -80°C for 10 days and followed a thawing process at 17°C. The rheological properties, bonding water contents and melting points of the gelatin/alginate hydrogel systems were changed with the addition of different amounts of DMSO. The microscopy analysis, (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrasodium bromide (MTT) and hematoxylin and eosin (HE) staining indicated that the cell numbers were progressively in a selected DMSO concentration range. With DMSO 5% (v/v) alone, the metabolic rate in the construct attained (81.3 ± 5.7)%. A synergistic effect was achieved with the combination of the DMSO/gelatin/alginate and dextran-40/gelatin/alginate hydrogel systems. These results indicated that the inclusion of DMSO and dextran-40 in the hydrogel could effectively enhance the cell preservation effects. This cryopreservation strategy holds the ability to be widely used in organ manufacturing techniques. [Reprinted with permission from Elsevier. Copyright, 2010].

Détails

  • Titre original : Incorporation of DMSO and dextran-40 into a gelatin/alginate hydrogel for controlled assembled cell cryopreservation.
  • Identifiant de la fiche : 30003665
  • Langues : Anglais
  • Source : Cryobiology - vol. 61 - n. 3
  • Date d'édition : 12/2010
  • DOI : http://dx.doi.org/10.1016/j.cryobiol.2010.10.161

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