Summary
A new cell cryopreservation strategy for cell-assembling constructs was proposed. With this strategy, different concentrations of dimethysulfoxide (DMSO) and dextran-40 were directly incorporated into the cell/gelatin/alginate systems, prototyped according to a predesigned structure, cryopreserved at -80°C for 10 days and followed a thawing process at 17°C. The rheological properties, bonding water contents and melting points of the gelatin/alginate hydrogel systems were changed with the addition of different amounts of DMSO. The microscopy analysis, (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrasodium bromide (MTT) and hematoxylin and eosin (HE) staining indicated that the cell numbers were progressively in a selected DMSO concentration range. With DMSO 5% (v/v) alone, the metabolic rate in the construct attained (81.3 ± 5.7)%. A synergistic effect was achieved with the combination of the DMSO/gelatin/alginate and dextran-40/gelatin/alginate hydrogel systems. These results indicated that the inclusion of DMSO and dextran-40 in the hydrogel could effectively enhance the cell preservation effects. This cryopreservation strategy holds the ability to be widely used in organ manufacturing techniques. [Reprinted with permission from Elsevier. Copyright, 2010].
Details
- Original title: Incorporation of DMSO and dextran-40 into a gelatin/alginate hydrogel for controlled assembled cell cryopreservation.
- Record ID : 30003665
- Languages: English
- Source: Cryobiology - vol. 61 - n. 3
- Publication date: 2010/12
- DOI: http://dx.doi.org/10.1016/j.cryobiol.2010.10.161
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Thawing; Organ; Cell; DMSO; Expérimentation; Cryopreservation
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Protocols for thawing and cryoprotectant diluti...
- Author(s) : ARMITAGE W. J., DALE M., ALEXANDER E. A.
- Date : 2005/02
- Languages : English
- Source: Cryobiology - vol. 50 - n. 1
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Effect of dimethyl sulfoxide on post-thaw viabi...
- Author(s) : YANG H., ZHAO H., ACKER J. P., et al.
- Date : 2005/10
- Languages : English
- Source: Cryobiology - vol. 51 - n. 2
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EFFECT OF DISSOLVED GASES ON FREEZE-THAW SURVIV...
- Author(s) : KRUUV J.
- Date : 1985
- Languages : English
- Source: Cryo-Letters - vol. 6 - n. 4
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Cryopreservation of strawberry cell suspension ...
- Author(s) : YONGJIE W., ENGELMANN F., FRATTARELLI A., DAMIANO C., WITHERS L. A.
- Date : 1997/09
- Languages : English
- Source: Cryo-Letters - vol. 18 - n. 5
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EFFECT OF RAPID ADDITION AND DILUTION OF DMSO A...
- Author(s) : KOJIMA T., SOMA T., OGURI N.
- Date : 1987
- Languages : English
- Source: Cryobiology - vol. 24 - n. 3
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