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Influence de la cryoconservation sur l'intégrité structurelle et fonctionnelle des membranes des cellules de cals embryogéniques de canne à sucre (Saccharum sp.).

Effect of cryopreservation on the structural and functional integrity of cell membranes of sugarcane (Saccharum sp.) embryogenic calluses.

Auteurs : MARTÍNEZ-MONTERO M. E., MORA N., QUIÑONES J., et al.

Type d'article : Article

Résumé

The authors investigated whether the differences consistently noted in survival and plantlet production between cryopreserved and non-cryopreserved, control sugarcane embryogenic calluses were related to modifications induced during cryopreservation in the structural and functional integrity of cell membranes. The evolution of electrolyte leakage, lipid peroxidation products and cell membrane protein contents was measured for 5 days after cryopreservation. Differences between control and frozen calluses were observed only during the first 2 or 3 days after freezing. It was not possible to link these differences with the differences in survival and plant production between control and cryopreserved calluses. Additional studies are thus needed to elucidate which biochemical factors, linked to survival and plantlet regeneration, are affected by cryopreservation.

Détails

  • Titre original : Effect of cryopreservation on the structural and functional integrity of cell membranes of sugarcane (Saccharum sp.) embryogenic calluses.
  • Identifiant de la fiche : 2003-2054
  • Langues : Anglais
  • Source : CryoLetters - vol. 23 - n. 4
  • Date d'édition : 07/2002
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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