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Cryoconservation des pousses de canne à sucre encapsulées : influence de la température et de la durée d'entreposage.

Cryopreservation of encapsulated sugarcane apices: effect of storage temperature and storage duration.

Auteurs : GONZÁLEZ-ARNAO M. T., URRA C., ENGELMANN F., et al.

Type d'article : Article

Résumé

Apices sampled on in vitro plantlets of sugarcane were cryopreserved using the encapsulation-dehydration technique, comprising pregrowth for 24 h in liquid medium with 0.75 M sucrose, desiccation to 20-25% moisture content (fresh weight basis) and rapid immersion in liquid nitrogen. Apices of one variety were stored for up to one year at -196, -70 or -25 °C, with or without previous transitory immersion in liquid nitrogen. Whereas the recovery percentage of apices stored at -196 °C remained high and constant throughout the experiment, recovery of apices conserved at -70 and -25 °C decreased, respectively. The recovery percentage of apices of four additional varieties remained stable throughout a 1 year storage period at -196 °C.

Détails

  • Titre original : Cryopreservation of encapsulated sugarcane apices: effect of storage temperature and storage duration.
  • Identifiant de la fiche : 2000-3210
  • Langues : Anglais
  • Source : Cryo-Letters - vol. 20 - n. 6
  • Date d'édition : 11/1999
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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