Share

IIR document

KrioBlast™, a platform for kinetic vitrification by “click” (hyperfast) cooling: applications for cryopreservation and cryobanking.

Number: pap. n. 11

Author(s) : KATKOV I. I., BOLYUKH V. F., LIU Y., et al.

Summary

Kinetic (very rapid) vitrification (K-VF) is a promising approach for cryopreservation (CP) of biological materials and cryobanking for reproductive and regenerative medicine, husbandry, and cryopreservation of wildlife genetic resources as it is simple, robust, and can achieve VF for practically any type of cells. Up to know, superfast K-VF methods require very small sample size and cannot avoid the Leidenfrost effect (LFE), which substantially impedes the rate of cooling. Here, we are reporting an entirely novel system “KrioBlast(TM)“ for hyper-fast K-VF (“click” cooling) of one-two order of magnitude larger samples. The system completely eliminates LFE and a need of potentially toxic and osmotically damaging vitrificants such as DMSO or ethylene glycol used in the current methods of VF. Preliminary experiments have shown feasibility and potentially very high survival of cryopreserved human spermatozoa and embryonic stem cells.

Available documents

Format PDF

Pages: 6 p.

Available

  • Public price

    20 €

  • Member price*

    Free

* Best rate depending on membership category (see the detailed benefits of individual and corporate memberships).

Details

  • Original title: KrioBlast™, a platform for kinetic vitrification by “click” (hyperfast) cooling: applications for cryopreservation and cryobanking.
  • Record ID : 30011203
  • Languages: English
  • Source: Cryogenics 2014. Proceedings of the 13th IIR International Conference: Prague, Czech Republic, April 7-11, 2014.
  • Publication date: 2014/04/07

Links


See other articles from the proceedings (51)
See the conference proceedings

Indexing