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Prévention de la transmission d'infection lors de la conservation et de l'entreposage à basse température de matériaux biologiques à des températures inférieures à -80 °C.

Prevention of infection transmission in low-temperature preservation and storage of biological materials below -80 °C.

Auteurs : MERICKA P., STEPÁNOVA V., BLÁHA M., et al.

Résumé

The potential risk of infection transmission during storage was evaluated in 40 patients and 5 donors undergoing collection of haemopoietic progenitor cells for transplantation. Serological tests included proof of retroviruses, hepatitis viruses, herpes viruses, syphilis and toxoplasmosis. Acute infection requiring treatment was found in 2 patients. Laboratory signs of active infection with CMV were detected in 6, with VZV in 2, with HSV in 2 and with toxoplasmosis in 1 patient. Although contamination during storage in liquid nitrogen was apparently caused by technical failures, the authors' results showed that the infection rate in patients undergoing collections was relatively high. Only bags from patients without any signs of acute infection can be stored in the liquid phase of nitrogen.

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Pages : 2002-1

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Détails

  • Titre original : Prevention of infection transmission in low-temperature preservation and storage of biological materials below -80 °C.
  • Identifiant de la fiche : 2003-3053
  • Langues : Anglais
  • Source : Cryogenics 2002. Proceedings of the 7th Cryogenics Conference
  • Date d'édition : 23/04/2002

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