Partager

Document IIF

Effet de la cryoconservation sur l'expression de gènes sans souches dans des cellules fœtales de foies de souris.

The effect of cryopreservation on the expression of stemness-genes in mouse fetal liver cells.

Numéro : pap. n. 62

Auteurs : BORYSOV P., DIMITROV A., OSTANKOV M., et al.

Résumé

Nowadays mesenchymal (MSCs) and hematopoetic (HSCs) stem cells of fetal liver (FL) are used in cell and tissue therapy. The important part of application of biological objects in clinical practice is cryopreservation. Stem cells self-renewal and pluripotency are controlled by transcription factors. The most famous are Nanog, Oct4 and SOX2. The analysis of stemness-genes (nanog, oct4 and sox2) state could give understanding of principles of cell genomic apparatus functioning after cryopreservation, and upgrade existing cryopreservation protocols. It has been demonstrated that our methods of isolation and cryopreservation allow saving high percentage of viable cells. The expression level of investigated genes in FL suspension and isolated MSCs and HSCs was different before and after cryopreservation. Thus, cryopreservation changes pattern of stemness-genes expression in studied fractions, which can affect their features in different ways.

Documents disponibles

Format PDF

Pages : 6 p.

Disponible

  • Prix public

    20 €

  • Prix membre*

    Gratuit

* meilleur tarif applicable selon le type d'adhésion (voir le détail des avantages des adhésions individuelles et collectives)

Détails

  • Titre original : The effect of cryopreservation on the expression of stemness-genes in mouse fetal liver cells.
  • Identifiant de la fiche : 30011194
  • Langues : Anglais
  • Source : Cryogenics 2014. Proceedings of the 13th IIR International Conference: Prague, Czech Republic, April 7-11, 2014.
  • Date d'édition : 07/04/2014

Liens


Voir d'autres communications du même compte rendu (51)
Voir le compte rendu de la conférence

Indexation