Partager

Propriétés mécaniques de la membrane du globule rouge humain à -15 °C

Mechanical properties of the human red blood cell membrane at -15°C.

Auteurs : THOM F.

Type d'article : Article

Résumé

The most common method for measuring the mechanical behaviour of the human red blood cell (RBC) membrane is micropipette aspiration, because it can be used to apply both a low uniaxial stress at a small part of the membrane or high two-axial stresses to the whole membrane. The elastic shear moduli and area changes of the human RBC published to date were calculated by means of this technique. However, a main drawback of the method is its impracticability at subzero temperatures. Experiments at below 0°C are of interest because it is at these temperatures that RBC lysis occurs during freezing and thawing after cryopreservation, via a mechanism that may be mechanical. A method for circumventing this limitation is deforming the cell membranes by applying an electric ac field to a supercooled suspension. In a previous study, the author applied this technique to human RBCs down to -15°C. In this technique, the electrical dimensions must be translated into those of mechanics. He provided a formula for these calculations, which demonstrated excellent concordance with known mechanical measurements at room temperature. Using this formula, the author has now calculated the shear moduli and stress-strain diagram for our deformation experiments at -15°C and present the results below. [Reprinted with permission from Elsevier. Copyright, 2009].

Détails

  • Titre original : Mechanical properties of the human red blood cell membrane at -15°C.
  • Identifiant de la fiche : 2009-2174
  • Langues : Anglais
  • Source : Cryobiology - vol. 59 - n. 1
  • Date d'édition : 08/2009
  • DOI : http://dx.doi.org/10.1016/j.cryobiol.2009.04.001

Liens


Voir d'autres articles du même numéro (2)
Voir la source

Indexation