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Viablité de tissu ovarien humain confirmé 5 ans après congélation avec formation spontanée de glace grâce à une culture de membrane chorio-allantoïque et l'auto-greffage.

Viability of human ovarian tissue confirmed 5 years after freezing with spontaneous ice formation by autografting and chorio-allantoic membrane culture.

Auteurs : ISACHENKO V., ORTH I., ISACHENKO E., et al.

Type d'article : Article, Synthèse

Résumé

To achieve optimal and uniform outcomes, slow cooling protocols for human ovarian tissues generally initiate ice formation at high sub-zero temperatures (-6 to -9°C). The aim of the study was to investigate the function of ovarian tissue that had unintentionally self seeded at -20°C during the freezing step, by examining its development following chicken embryonic chorioallantoic membrane (CAM) grafting and after transplantation back to the patient. Ovarian tissue was frozen in 6% (v/v) dimethyl sulfoxide, 6% (v/v) ethylene glycol and 0.15 M sucrose which had self-seeded at -20°C. Five years after cryopreservation, 8 pieces were thawed and transplanted back to the patient. Two small (1x 2 x 1 mm) pieces of this thawed tissue were cultured in a CAM-system for 5days to assess the tissue viability. The autografted ovarian tissue re-established spontaneous menstrual bleeding within five months and raised serum 17-b Estradiol from 19 to 330 pg/ml. Ultrasound revealed a dominant follicle at the site of the transplanted tissue in the follicular phase after the menstrual bleed. Analysis of the CAM cultured tissue established that 88% of the primordial follicles are degenerated and there was limited in growth of blood vessels. In conclusion, in spite of the damage caused by the cryopreservation with spontaneous ice-formation the viability could be confirmed by CAM culture and the restoration of ovarian function after auto-transplantation.

Détails

  • Titre original : Viability of human ovarian tissue confirmed 5 years after freezing with spontaneous ice formation by autografting and chorio-allantoic membrane culture.
  • Identifiant de la fiche : 30013188
  • Langues : Anglais
  • Source : Cryobiology - vol. 66 - n. 3
  • Date d'édition : 06/2013
  • DOI : http://dx.doi.org/10.1016/j.cryobiol.2013.02.003

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