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Viscosités de la solution ternaire de chlorure de diméthyle sulfoxyde/eau/soude à des températures inférieures à zéro et leur application dans la cryoconservation.

Viscosities of the ternary solution dimethyl sulfoxide/water/sodium chloride at subzero temperatures and their application in cryopreservation.

Auteurs : ZHANG S., YU X., CHEN Z., et al.

Type d'article : Article

Résumé

Vitrification is considered as the most promising method for long-term storage of tissues and organs. An effective way to reduce the accompanied cryoprotectant (CPA) toxicity, during CPA addition/removal, is to operate at low temperatures. The permeation process of CPA into/out of biomaterials is affected by the viscosity of CPA solution, especially at low temperatures. The objective of the present study is to measure the viscosity of the ternary solution, dimethyl sulfoxide (Me2SO)/water/sodium chloride (NaCl), at low temperatures and in a wide range of concentrations. A rotary viscometer coupled with a low temperature thermostat bath was used. The measurement was carried out at temperatures from -10 to -50°C. The highest mass fraction of Me2SO was 75% (w/w) and the lowest mass fraction of Me2SO was the value that kept the solution unfrozen at the measurement temperature. The concentration of NaCl was kept as a constant [0.85% (w/w), the normal salt content of extracellular fluids]. The Williams–Landel–Ferry (WLF) model was employed to fit the obtained viscosity data. As an example, the effect of solution viscosity on modeling the permeation of Me2SO into articular cartilage was qualitatively analyzed.

Détails

  • Titre original : Viscosities of the ternary solution dimethyl sulfoxide/water/sodium chloride at subzero temperatures and their application in cryopreservation.
  • Identifiant de la fiche : 30013394
  • Langues : Anglais
  • Source : Cryobiology - vol. 66 - n. 2
  • Date d'édition : 04/2013
  • DOI : http://dx.doi.org/10.1016/j.cryobiol.2013.01.005

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