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Altérations et protection de la peau porcine fendue après refroidissement suivi de réchauffement rapide.

Injury and protection in split-thickness skin after very rapid cooling and warming.

Auteurs : ZIEGER M. A. J., TREDGET E. E., SYKES B. D., MCGANN L. E.

Type d'article : Article

Résumé

Recovery of aerobic activity increased in a concentration-dependent manner, and reached 100% after a 10-minute exposure to 2 moles glycerol at 4 deg C or 3 minutes at 22 deg C, for an untake of 1.1 mole glycerol. Light micrographs of freeze-substituted skin showed a glycerol-dependent decrease in the nucleation and growth of ice in the dermis and epidermis after rapid cooling. A 5-minute exposure to 2 moles glycerol at 22 deg C resulted in the elimination of all observable epidermal ice, except for extremely small ice crystals in the intercellular spaces and in few nuclei, and complete preservation of the fibrous structure of dermal collagen bundles. This cryoprotective mechanism has the potential to offer complete protection of both dermal and viable epidermal targets of freeze/thaw injury and may be applicable to other thin, membranous tissues.

Détails

  • Titre original : Injury and protection in split-thickness skin after very rapid cooling and warming.
  • Identifiant de la fiche : 1998-3194
  • Langues : Anglais
  • Source : Cryobiology - vol. 35 - n. 1
  • Date d'édition : 08/1997

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