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Comparaison de quatre méthodes de cryoconservation des méristèmes de banane (Musa spp.).

A comparison of four methods of cryopreservation of meristems in banana (Musa spp.).

Auteurs : AGRAWAL A., SWENNEN R., PANIS B.

Type d'article : Article

Résumé

To establish an improved protocol for the cryopreservation of banana (Musa spp.), a fast-freeze/fast-thaw method was applied and compared with three existing cryogenic procedures: simple freezing, vitrification of proliferating meristems and vitrification of individual meristems. The average post-thaw shoot regeneration of the fast-freeze/fast-thaw protocol was 51.8% and was superior to the existing protocols which were 12.5, 39.2 and 42.8%, respectively. Other factors such as time and ease of operation were considered for each method. It was found that the fast-freeze/fast-thaw technique was the best of the four methods tested for the cryopreservation of in vitro banana meristems in the genotype Robusta (AAA).

Détails

  • Titre original : A comparison of four methods of cryopreservation of meristems in banana (Musa spp.).
  • Identifiant de la fiche : 2005-0437
  • Langues : Anglais
  • Source : CryoLetters - vol. 25 - n. 2
  • Date d'édition : 03/2004

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