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A comparison of four methods of cryopreservation of meristems in banana (Musa spp.).

Author(s) : AGRAWAL A., SWENNEN R., PANIS B.

Type of article: Article

Summary

To establish an improved protocol for the cryopreservation of banana (Musa spp.), a fast-freeze/fast-thaw method was applied and compared with three existing cryogenic procedures: simple freezing, vitrification of proliferating meristems and vitrification of individual meristems. The average post-thaw shoot regeneration of the fast-freeze/fast-thaw protocol was 51.8% and was superior to the existing protocols which were 12.5, 39.2 and 42.8%, respectively. Other factors such as time and ease of operation were considered for each method. It was found that the fast-freeze/fast-thaw technique was the best of the four methods tested for the cryopreservation of in vitro banana meristems in the genotype Robusta (AAA).

Details

  • Original title: A comparison of four methods of cryopreservation of meristems in banana (Musa spp.).
  • Record ID : 2005-0437
  • Languages: English
  • Source: CryoLetters - vol. 25 - n. 2
  • Publication date: 2004/03

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