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Cryoconservation des bourgeons axillaires d'Eucalyptus grandis x Eucalyptus camaldulensis hybride.

Cryopreservation of axillary buds of a Eucalyptus grandis x Eucalyptus camaldulensis hybrid.

Auteurs : BLAKESLEY D., KIERNAN R. J.

Type d'article : Article

Résumé

The authors developed methodology for the cryopreservation of axillary buds from an in vitro hybrid Eucalyptus grandis (W. Hill ex Maiden.) x Eucalyptus camaldulensis (Dehnnh), maintained for use in a genetic modification programme. Axillary buds were encapsulated in an alginate gel, precultured on media containing elevated levels of sucrose, or a combination of sucrose and glycerol. Encapsulated buds were then dehydrated by evaporation prior to a two-step freezing process in liquid nitrogen. Eighteen percent of shoot explants survived freezing when sucrose alone was used as a protectant against dehydration and cryopreservation. Significantly higher survival (49%) was obtained with the incorporation of glycerol into the protocol. Following cryopreservation, shoots appeared to develop normally.

Détails

  • Titre original : Cryopreservation of axillary buds of a Eucalyptus grandis x Eucalyptus camaldulensis hybrid.
  • Identifiant de la fiche : 2002-0259
  • Langues : Anglais
  • Source : CryoLetters - vol. 22 - n. 1
  • Date d'édition : 01/2001
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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