Share

Cryopreservation of axillary buds of a Eucalyptus grandis x Eucalyptus camaldulensis hybrid.

Author(s) : BLAKESLEY D., KIERNAN R. J.

Type of article: Article

Summary

The authors developed methodology for the cryopreservation of axillary buds from an in vitro hybrid Eucalyptus grandis (W. Hill ex Maiden.) x Eucalyptus camaldulensis (Dehnnh), maintained for use in a genetic modification programme. Axillary buds were encapsulated in an alginate gel, precultured on media containing elevated levels of sucrose, or a combination of sucrose and glycerol. Encapsulated buds were then dehydrated by evaporation prior to a two-step freezing process in liquid nitrogen. Eighteen percent of shoot explants survived freezing when sucrose alone was used as a protectant against dehydration and cryopreservation. Significantly higher survival (49%) was obtained with the incorporation of glycerol into the protocol. Following cryopreservation, shoots appeared to develop normally.

Details

  • Original title: Cryopreservation of axillary buds of a Eucalyptus grandis x Eucalyptus camaldulensis hybrid.
  • Record ID : 2002-0259
  • Languages: English
  • Source: CryoLetters - vol. 22 - n. 1
  • Publication date: 2001/01
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (3)
See the source

Indexing