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Cryoconservation des cellules mésenchymateuses stromales par vitrification dans des solutions à plusieurs composants.

Cryopreservation of mesenchymal stromal cells by vitrification in multicomponent solutions.

Numéro : pap. n. 087

Auteurs : PETRENKO A. Ju., TRUFANOVA N. A., PETRENKO Ju. A.

Résumé

Due to capacity for differentiation into several cell lineages mesenchymal stromal cells (MSCs) are promising cellular components for cell therapy and tissue engineering that causes the need of cryopreservation of these cells. In the study combinations of cryoprotectants that allow avoiding ice formation during rapid cooling and rewarming as well as maintaining viability and unique properties of MSCs were discovered. The vitrification solutions consisted of dimethylsulfoxide, ethylene glycol, 1,2-propandiol and sucrose. Two vitrification multi-component solutions called DEPS-1 and DEPS-2, which prevented ice crystal formation in 2 ml cryovials during cooling and thawing were chosen for MSCs vitrification. After vitrification in DEPS-1 MSCs demonstrated higher viability and attachment rates than in DEPS-2. MSCs cryopreserved by vitrification in DEPS-1 were able to clonogenic growth and multilineage differentiation during culture. The vitrification protocol using DEPS-1 as CPA was adapted for cryopreservation of MSCs encapsulated in alginate microspheres.

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Pages : 254-259

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Détails

  • Titre original : Cryopreservation of mesenchymal stromal cells by vitrification in multicomponent solutions.
  • Identifiant de la fiche : 30006657
  • Langues : Anglais
  • Source : Cryogenics 2012. Proceedings of the 12th IIR International Conference: Dresden, Germany, September 11-14, 2012.
  • Date d'édition : 11/09/2012

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