Partager

Document IIF

Cryoconservation par vitrification : principes, méthodes et dispositifs thermodynamiques de base.

Cryopreservation by vitrification: basic thermodynamic principals, methods and devices.

Numéro : pap. n. 26

Auteurs : KATKOV I. I., BOLYUKH V. F.

Résumé

There are 5 basics ways of achieving long-term storage, which ALL essentially lead to vitrification of cells, namely 1. Slow freezing, 2. Equilibrium ice-free vitrification (E-VF) with high concentration of vitrificants and relatively moderate speed of cooling and rewarming, 3. Kinetic intracellular ice-free vitrification (K-VF) with very rapid rates of cooling and rewarming and low to none concentration of exogenous vitrificants, 4. Freeze-drying (lyophilization), and 5. Vacuum/air flow drying at temperatures above zero degree of Celsius (xeropreservation). In this presentation, we will focus on the kinetic vs. equilibrium vitrification. We will compare mechanisms, analyze phase diagrams, emphasize the role of the Leidenfrost effect (LFE) and ways of reducing up to full eliminating LFE. And finally, we will introduce our novel hyper-fast scalable cooling devices KrioBlast™ and VitriPlunger™ and briefly discuss the promising results on K-VF of human sperm, embryonic stem cells and insulin-producing cells using those systems.

Documents disponibles

Format PDF

Pages : 6

Disponible

  • Prix public

    20 €

  • Prix membre*

    Gratuit

* meilleur tarif applicable selon le type d'adhésion (voir le détail des avantages des adhésions individuelles et collectives)

Détails

  • Titre original : Cryopreservation by vitrification: basic thermodynamic principals, methods and devices.
  • Identifiant de la fiche : 30021702
  • Langues : Anglais
  • Source : Cryogenics 2017. Proceedings of the 14th IIR International Conference: Dresden, Germany, Mai 15-19, 2017.
  • Date d'édition : 15/05/2017
  • DOI : http://dx.doi.org/10.18462/iir.cryo.2017.0026

Liens


Voir d'autres communications du même compte rendu (71)
Voir le compte rendu de la conférence

Indexation