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Cryoconservation des plaquettes sanguines à l'aide d'adrénaline et de DMSO en tant que cryoprotecteurs.

Platelet cryopreservation using a combination of epinephrine and dimethyl sulfoxide as cryoprotectants.

Auteurs : XIAO H., HARVEY K., LABARRERE C. A., et al.

Type d'article : Article

Résumé

Current methods of platelet storage are unsatisfactory because of the short shelf life of platelets and the rapid loss of platelet viability. The authors developed a cryopreservation method that results in less damage from freezing and higher recovered function. Platelets were cryopreserved using epinephrine (EPN) and dimethyl sulfoxide (DMSO) as cryoprotectant. The response of platelets to agonists was studied by flow cytometry and aggregation tests. Cryopreserving platelets with DMSO decreased platelet annexin V binding due to freezing. The combination of EPN with enhanced DMSO cryoprotection and decreased platelet microparticle generation, suggesting increased platelet integrity. Platelet cryopreservation with an DMSO/EPN combination also increased platelet aggregability. The addition of EPN as combined cryoprotectant thus improves the quality of DMSO-frozen platelets. As a method of aggregation of cryopreserved platelets, this method is comparable to that of normal fresh platelets and may improve platelet-transfusion conditions.

Détails

  • Titre original : Platelet cryopreservation using a combination of epinephrine and dimethyl sulfoxide as cryoprotectants.
  • Identifiant de la fiche : 2001-2515
  • Langues : Anglais
  • Source : Cryobiology - vol. 41 - n. 2
  • Date d'édition : 09/2000

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