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Cryoconservation des plaquettes utilisant une concentration de DMSO réduite et des effecteurs deuxièmes messagers en tant que solution de cryoconservation.

Platelet cryopreservation using a reduced DMSO concentration and second-messenger effectors as cryopreserving solution.

Auteurs : LOZANO M. L., RIVERA J., CORRAL J., et al.

Type d'article : Article

Résumé

Cryopreservation of platelets is of great interest since it could extend to years the shelf life of therapeutic platelet concentrates (PCs) and facilitate stockpiling and inventory control in blood banking. The authors compared the cryopreservation of PCs by the standard method using 6% DMSO as cryoprotectant with the method of freezing employing low concentrations of DMSO (2%) plus ThromboSol, a mixture of second-messenger effectors that protect platelets from cold damage. PC pools were treated either with 6% DMSO or with ThromboSol and 2% DMSO and then placed directly in a -80 °C freezer or in the vapour phase of a liquid nitrogen freezer (-120 °C). After storage for 1 week or for 3 months, samples were removed, thawed and analysed. Measurements included cell recovery, biochemical parameters, membrane glycoproteins (GPs), platelet aggregation, and binding of radiolabelled von Willebrand factor (vWF) and fibrinogen. PCs cryopreserved with ThromboSol and 2% DMSO displayed a platelet recovery (90%) equivalent to those frozen with 6% DMSO. Both methods promoted comparable impairment of the reactivity of platelets to thrombin, aggregation and binding of fibrinogen and vWF, compared to that of fresh platelets. Cryopreservation of PCs using reduced DMSO concentration and ThromboSol yields platelets with in vitro functional characteristics equivalent to those of cells frozen with the conventional method using 6% DMSO.

Détails

  • Titre original : Platelet cryopreservation using a reduced DMSO concentration and second-messenger effectors as cryopreserving solution.
  • Identifiant de la fiche : 2000-2235
  • Langues : Anglais
  • Source : Cryobiology - vol. 39 - n. 1
  • Date d'édition : 08/1999

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