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Cryoconservation des pousses d'agrumes en utilisant la vitrification : l'effet d'une forme réduite de glutathion.

Cryopreservation of shoot-tips of citrus using vitrification: the effect of a reduced form of glutathion.

Auteurs : WANG Z. C., DENG X. X.

Type d'article : Article

Résumé

A commercial citrus scion cultivar '439' tangor was used to investigate whether GSH (reduced form of glutathione) could improve survival following a vitrification. The optimal pre-growth treatment was 3 days pre-culture on basal pre-culture medium (BPM: MT basal medium containing 0.5 mol/L glycerol and 5% sucrose at pH 5.8). GSH of 40 mg/L in the pre-culture medium improved shoot tip survival after cryopreservation. GSH in the recovery medium also improved survival, with 10 mg/L proving optimal. GSH of 40 mg/L in the loading and vitrification solutions also improved survival. The optimal cryopreservation protocol was successfully applied to 12 other citrus cultivars.

Détails

  • Titre original : Cryopreservation of shoot-tips of citrus using vitrification: the effect of a reduced form of glutathion.
  • Identifiant de la fiche : 2004-2512
  • Langues : Anglais
  • Source : Cryo-Letters - vol. 25 - n. 1
  • Date d'édition : 01/2004
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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