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Cryopreservation of shoot-tips of citrus using vitrification: the effect of a reduced form of glutathion.

Author(s) : WANG Z. C., DENG X. X.

Type of article: Article

Summary

A commercial citrus scion cultivar '439' tangor was used to investigate whether GSH (reduced form of glutathione) could improve survival following a vitrification. The optimal pre-growth treatment was 3 days pre-culture on basal pre-culture medium (BPM: MT basal medium containing 0.5 mol/L glycerol and 5% sucrose at pH 5.8). GSH of 40 mg/L in the pre-culture medium improved shoot tip survival after cryopreservation. GSH in the recovery medium also improved survival, with 10 mg/L proving optimal. GSH of 40 mg/L in the loading and vitrification solutions also improved survival. The optimal cryopreservation protocol was successfully applied to 12 other citrus cultivars.

Details

  • Original title: Cryopreservation of shoot-tips of citrus using vitrification: the effect of a reduced form of glutathion.
  • Record ID : 2004-2512
  • Languages: English
  • Source: Cryo-Letters - vol. 25 - n. 1
  • Publication date: 2004/01
  • Document available for consultation in the library of the IIR headquarters only.

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