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Double impact d'une protéine antigel sur la cryoconservation des cellules embryogéniques de riz (Oryza sativa L.) en suspension.

The dual effect of antifreeze protein on cryopreservation of rice (Oryza sativa L.) embryogenic suspension cells.

Auteurs : WANG J. H., BIAN H. W., ZHANG Y. X., et al.

Type d'article : Article

Résumé

The effects of fish antifreeze protein AFP- on cryopreservation of rice suspension cells by three different protocols were investigated. During the two-step method, AFP- at 0.01 mg/ml significantly lowered the viability of both precultured and non-precultured cells. During the vitrification method, AFP- at 0.2 mg/ml improved the viability of suboptimally thawed cells; however, much higher doses of this protein (10mg/ml) attenuated cell viability. During rapid freezing of rice cells in the solutions with relatively high (but non-vitrifying) concentrations of cryoprotectant, AFP- displayed protective action in the higher concentrated cryoprotectants and detrimental effect in more dilute ones. It was concluded that, depending on a number of factors discussed, both positive and negative effects could be observed during application of AFP to cryopreservation of rice cells. The possible mechanism of this dual character was discussed.

Détails

  • Titre original : The dual effect of antifreeze protein on cryopreservation of rice (Oryza sativa L.) embryogenic suspension cells.
  • Identifiant de la fiche : 2004-1971
  • Langues : Anglais
  • Source : Cryo-Letters - vol. 22 - n. 3
  • Date d'édition : 05/2001
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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