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Précroissance-déshydratation : une procédure simple et efficace pour la cryoconservation des cellules embryogéniques du riz en suspension.

Pregrowth-desiccation: a simple and efficient procedure for the cryopreservation of rice embryogenic suspension cells.

Auteurs : ZHANG Y. X., WANG J. H., BIAN H. W., et al.

Type d'article : Article

Résumé

Rice embryogenic suspension cells were cryopreserved by a pregrowth-desiccation procedure. Cells were precultured in liquid AA medium containing 0.175 mol/L sucrose for 3 days and then in liquid AA medium containing 0.4 mol/L sorbitol for 1 day. After air-drying for about 20 h to a water content of 10%, the cells were placed into cryotubes and quenched into liquid nitrogen. A survival rate of 96 plus or minus 6% (TTC reduction assay) or 100% (cell clump regrowth) was achieved. Cryostored cells revived very quickly during recovery culture and retained the ability to regenerate fertile plants. Air-drying, a method usually employed in cryopreservation of seeds of shoot tips, can thus be used as a simple and efficient procedure for the cryopreservation of precultured rice suspension cells.

Détails

  • Titre original : Pregrowth-desiccation: a simple and efficient procedure for the cryopreservation of rice embryogenic suspension cells.
  • Identifiant de la fiche : 2002-2147
  • Langues : Anglais
  • Source : CryoLetters - vol. 22 - n. 4
  • Date d'édition : 07/2001
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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