Partager

Effet des concentrations réduites en glycérol et de diverses macromolécules sur la cryoconservation des embryons de souris et de boeuf.

Effect of reduced concentrations of glycerol and various macromolecules on the cryopreservation of mouse and cattle embryos.

Auteurs : PALASZ A. T., THUNDATHIL J., FUENTE J. de la, et al.

Type d'article : Article

Résumé

The effect of different macromolecules [bovine serum albumin (SBA), Pluronic F-68, (ET surfactant), or sodium hyaluronate (SH)] on postthaw survival of mouse morulae and in vivo- and in vitro-derived bovine blastocysts frozen in 10, 5, or 1% glycerol solutions was investigated. Embryos were equilibrated with cryoprotectant solution at 25 °C for 10 min, seeded at -5 °C, cooled at 0.5 °C/min to -35 °C, and plunged into liquid nitrogen. Embryos were thawed in a 35 °C water bath, glycerol was removed with 0.6 M sucrose at 25 °C for 5 min, and postthaw viability was evaluated after 1, 24, and 48 h. The addition of BSA supplementation improved postthaw survival of mouse morulae frozen in 5% glycerol, but not in 10% glycerol. All three macromolecular supplements were effective in increasing survival of mouse morulae in 5% glycerol but only BSA and SH were effective in increasing postthaw survival of in vivo- and in vitro-derived bovine blastocysts. None of the macromolecular supplements improved postthaw survival of embryos frozen in 1% glycerol.

Détails

  • Titre original : Effect of reduced concentrations of glycerol and various macromolecules on the cryopreservation of mouse and cattle embryos.
  • Identifiant de la fiche : 2001-2951
  • Langues : Anglais
  • Source : Cryobiology - vol. 41 - n. 1
  • Date d'édition : 08/2000

Liens


Voir d'autres articles du même numéro (3)
Voir la source

Indexation