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Effect of reduced concentrations of glycerol and various macromolecules on the cryopreservation of mouse and cattle embryos.

Author(s) : PALASZ A. T., THUNDATHIL J., FUENTE J. de la, et al.

Type of article: Article

Summary

The effect of different macromolecules [bovine serum albumin (SBA), Pluronic F-68, (ET surfactant), or sodium hyaluronate (SH)] on postthaw survival of mouse morulae and in vivo- and in vitro-derived bovine blastocysts frozen in 10, 5, or 1% glycerol solutions was investigated. Embryos were equilibrated with cryoprotectant solution at 25 °C for 10 min, seeded at -5 °C, cooled at 0.5 °C/min to -35 °C, and plunged into liquid nitrogen. Embryos were thawed in a 35 °C water bath, glycerol was removed with 0.6 M sucrose at 25 °C for 5 min, and postthaw viability was evaluated after 1, 24, and 48 h. The addition of BSA supplementation improved postthaw survival of mouse morulae frozen in 5% glycerol, but not in 10% glycerol. All three macromolecular supplements were effective in increasing survival of mouse morulae in 5% glycerol but only BSA and SH were effective in increasing postthaw survival of in vivo- and in vitro-derived bovine blastocysts. None of the macromolecular supplements improved postthaw survival of embryos frozen in 1% glycerol.

Details

  • Original title: Effect of reduced concentrations of glycerol and various macromolecules on the cryopreservation of mouse and cattle embryos.
  • Record ID : 2001-2951
  • Languages: English
  • Source: Cryobiology - vol. 41 - n. 1
  • Publication date: 2000/08

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