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Estimation de la concentration de DMSO pour la cryoconservation de cellule en état d'adhésion.

Estimation of DMSO concentration for cell cryopreservation on adhesion state.

Numéro : pap. n. 503

Auteurs : SEKINE H., OTAKI M., HAYASHI M., et al.

Résumé

Cryopreservation in the cell-adhesion state was investigated for the development of useful and easier handling technique of cell culture. In the present study, we used dimethyl sulfoxide (DMSO) as a cryoprotective agent and determined the suitable DMSO concentration for cryopreservation of HepG2 cells in the cell-adhesion state attached to glass and polydimethylsiloxane (PDMS) matrices coated with poly-Llysine. As a result, the rate of cell adhesion to PDMS matrix remained constant irrespective of the DMSO concentration between 10 v/v% and 30 v/v%. In the case of glass matrix, on the other hand, the rate of cell adhesion increased with the DMSO concentration. Cell viability was the highest at 20 v/v% DMSO concentration. DMSO concentration of 30 v/v%, however, had a cytotoxic effect on cell viability. Thus, 20 v/v% DMSO concentration was found to be the most suitable for cryopreservation of HepG2 cells in the adhesion state.

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Pages : 7 p.

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  • Prix public

    20 €

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    Gratuit

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Détails

  • Titre original : Estimation of DMSO concentration for cell cryopreservation on adhesion state.
  • Identifiant de la fiche : 30015965
  • Langues : Anglais
  • Source : Proceedings of the 24th IIR International Congress of Refrigeration: Yokohama, Japan, August 16-22, 2015.
  • Date d'édition : 16/08/2015
  • DOI : http://dx.doi.org/10.18462/iir.icr.2015.0503

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