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Etude sur la cryoconservation des tissus ovariens de souris par vitrification.

[In Chinese. / En chinois.]

Auteurs : WANG Y., HAN Y., WANG J., et al.

Type d'article : Article

Résumé

The research on cryopreservation of mouse ovary tissues was performed to explore suitable vitrification solutions and preservation methods. Ovary tissues of 3-week old mouse were dissected and cut into pieces, and then they were cryopreserved with different vitrification solutions. The tissues were kept at -196°C for one week and then reheated. The best vitrification solution was selected according to the histological observation on the tissue slices. The reheated ovary tissues were autotransplanted into the kidney capsules. Two weeks later, the autotransplanted tissues were taken out for evaluation. RT-PCR was used to detect AMH gene and KL gene. The results show that vitrification solution composed of infiltrative agents with high concentration could yield excellent effect on preservation of mouse ovary tissues. Our conclusion is that vitrification of mouse ovary may be feasible and detection of gene expression could be applied to validate the quality of cryopreservation.

Documents disponibles

Format PDF

Pages : 28-32

Disponible

  • Prix public

    20 €

  • Prix membre*

    15 €

* meilleur tarif applicable selon le type d'adhésion (voir le détail des avantages des adhésions individuelles et collectives)

Détails

  • Titre original : [In Chinese. / En chinois.]
  • Identifiant de la fiche : 2008-1387
  • Langues : Chinois
  • Source : Journal of Refrigeration - vol. 29 - n. 119
  • Date d'édition : 02/2008

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