Share

Study on cryopreservation of mouse ovary tissues by vitrification.

[In Chinese. / En chinois.]

Author(s) : WANG Y., HAN Y., WANG J., et al.

Type of article: Article

Summary

The research on cryopreservation of mouse ovary tissues was performed to explore suitable vitrification solutions and preservation methods. Ovary tissues of 3-week old mouse were dissected and cut into pieces, and then they were cryopreserved with different vitrification solutions. The tissues were kept at -196°C for one week and then reheated. The best vitrification solution was selected according to the histological observation on the tissue slices. The reheated ovary tissues were autotransplanted into the kidney capsules. Two weeks later, the autotransplanted tissues were taken out for evaluation. RT-PCR was used to detect AMH gene and KL gene. The results show that vitrification solution composed of infiltrative agents with high concentration could yield excellent effect on preservation of mouse ovary tissues. Our conclusion is that vitrification of mouse ovary may be feasible and detection of gene expression could be applied to validate the quality of cryopreservation.

Available documents

Format PDF

Pages: 28-32

Available

  • Public price

    20 €

  • Member price*

    15 €

* Best rate depending on membership category (see the detailed benefits of individual and corporate memberships).

Details

  • Original title: [In Chinese. / En chinois.]
  • Record ID : 2008-1387
  • Languages: Chinese
  • Source: Journal of Refrigeration - vol. 29 - n. 119
  • Publication date: 2008/02

Links


See other articles in this issue (5)
See the source

Indexing