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La cryoconservation de complexes ovocyte-cumulus bovins immatures altère la maturation in vitro en perturbant les jonctions intercellulaires.

Cryopreservation of immature bovine cumulus-oocyte complexes impairs in vitro maturation by disrupting the junctions between the cells.

Numéro : 0085

Auteurs : YURCHUK T., LIKSZO P., LUKASIK K., FALKO O., PETRUSHKO M., SKARZYNSKI D. J.

Résumé

Although cryopreservation of immature oocytes has many benefits for both female fertility preservation and biodiversity conservation, methods still need improvement. The aim of this study was to elucidate which cryobiological changes could affect maturation of bovine cumulus-oocyte complexes (COCs)
Immature bovine COCs were randomly divided into two groups: 1- fresh immCOCs and 2 - cryopreserved immCOCs by vitrification. The number of transzonal projection (TZP) and RNA expression of connexins (Cx) 37, 43, DNMT3a, BAX, BCL2 were evaluated in study groups after in vitro maturation (IVM). These results were compared with maturation, and blastocyst development rates. Our findings showed that cryopreservation decreases the number of TZP and induces significant changes in expression of gap-junction proteins. It also suppresses epigenetic changes normally activated during IVM in fresh COCs. Taken together, these changes led to a dramatic decrease in the rate of IVM and the development of the embryo. Thus, future improvement of the cryopreservation protocol for immCOCs should focused on restoring of the connections between the CCs and oocytes.

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Format PDF

Pages : 6 p.

Disponible

  • Prix public

    20 €

  • Prix membre*

    Gratuit

* meilleur tarif applicable selon le type d'adhésion (voir le détail des avantages des adhésions individuelles et collectives)

Détails

  • Titre original : Cryopreservation of immature bovine cumulus-oocyte complexes impairs in vitro maturation by disrupting the junctions between the cells.
  • Identifiant de la fiche : 30033909
  • Langues : Anglais
  • Sujet : Technologie
  • Source : Cryogenics 2025. Proceedings of the 18th IIR International Conference on Cryogenics, Prague, Czech Republic, 7-11 April 2025.
  • Date d'édition : 07/04/2025
  • DOI : http://dx.doi.org/10.18462/iir.cryo.2025.0085

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