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Cryopreservation of immature bovine cumulus-oocyte complexes impairs in vitro maturation by disrupting the junctions between the cells.
Number: 0085
Author(s) : YURCHUK T., LIKSZO P., LUKASIK K., FALKO O., PETRUSHKO M., SKARZYNSKI D. J.
Summary
Although cryopreservation of immature oocytes has many benefits for both female fertility preservation and biodiversity conservation, methods still need improvement. The aim of this study was to elucidate which cryobiological changes could affect maturation of bovine cumulus-oocyte complexes (COCs)
Immature bovine COCs were randomly divided into two groups: 1- fresh immCOCs and 2 - cryopreserved immCOCs by vitrification. The number of transzonal projection (TZP) and RNA expression of connexins (Cx) 37, 43, DNMT3a, BAX, BCL2 were evaluated in study groups after in vitro maturation (IVM). These results were compared with maturation, and blastocyst development rates. Our findings showed that cryopreservation decreases the number of TZP and induces significant changes in expression of gap-junction proteins. It also suppresses epigenetic changes normally activated during IVM in fresh COCs. Taken together, these changes led to a dramatic decrease in the rate of IVM and the development of the embryo. Thus, future improvement of the cryopreservation protocol for immCOCs should focused on restoring of the connections between the CCs and oocytes.
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Details
- Original title: Cryopreservation of immature bovine cumulus-oocyte complexes impairs in vitro maturation by disrupting the junctions between the cells.
- Record ID : 30033909
- Languages: English
- Subject: Technology
- Source: Cryogenics 2025. Proceedings of the 18th IIR International Conference on Cryogenics, Prague, Czech Republic, 7-11 April 2025.
- Publication date: 2025/04/07
- DOI: http://dx.doi.org/10.18462/iir.cryo.2025.0085
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