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La cryoconservation du sperme à -150 °C : une solution possible ?

Is sperm cryopreservation at -150 °C a feasible alternative?

Auteurs : MEDRANO A., CABRERA F., GONZÁLEZ F., et al.

Type d'article : Article

Résumé

A series of experiments was carried out to validate a -150 °C ultra-low temperature freezer prior to possible use to freeze and store semen. Crude sample handling was simulated to see whether temperature of stored samples was maintained within a safe range, and the freezing point and latent heat of fusion plateau of a semen extender were monitored. Buck semen was (i) frozen in liquid nitrogen and stored in the ultra-low freezer, (ii) frozen and stored in the ultra-low freezer, and (iii) frozen and stored in liquid nitrogen, to compare sperm cryosurvival between freezing methods. Semen stored either at -150 °C or at -196 °C cryosurvived similarly after 2 days and after 2 months of cryopreservation.

Détails

  • Titre original : Is sperm cryopreservation at -150 °C a feasible alternative?
  • Identifiant de la fiche : 2003-2052
  • Langues : Anglais
  • Source : CryoLetters - vol. 23 - n. 3
  • Date d'édition : 05/2002
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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