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Is sperm cryopreservation at -150 °C a feasible alternative?

Author(s) : MEDRANO A., CABRERA F., GONZÁLEZ F., et al.

Type of article: Article

Summary

A series of experiments was carried out to validate a -150 °C ultra-low temperature freezer prior to possible use to freeze and store semen. Crude sample handling was simulated to see whether temperature of stored samples was maintained within a safe range, and the freezing point and latent heat of fusion plateau of a semen extender were monitored. Buck semen was (i) frozen in liquid nitrogen and stored in the ultra-low freezer, (ii) frozen and stored in the ultra-low freezer, and (iii) frozen and stored in liquid nitrogen, to compare sperm cryosurvival between freezing methods. Semen stored either at -150 °C or at -196 °C cryosurvived similarly after 2 days and after 2 months of cryopreservation.

Details

  • Original title: Is sperm cryopreservation at -150 °C a feasible alternative?
  • Record ID : 2003-2052
  • Languages: English
  • Source: CryoLetters - vol. 23 - n. 3
  • Publication date: 2002/05
  • Document available for consultation in the library of the IIR headquarters only.

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