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La diffusivité thermique efficace comme indice d'évaluation de l'état des tissus artificiels cryoconservés.

Effective thermal diffusivity as an index for evaluation of the state in cryopreserved artificial tissue.

Auteurs : OKANIWA K., UJIHIRA M., MATSUMURA Y., et al.

Type d'article : Article

Résumé

Estimated intracellular effective thermal diffusivity (ETD) was used to evaluate the viability of cryopreserved biological tissue. Artificial tissues were prepared using human fibroblasts cultured for 2 days in a collagen sponge. The artificial tissue was frozen at cooling rates of 30 to 700°C/min from 4 to -196°C in culture medium containing propylene glycol. The temperature of the artificial tissue was then increased stepwise from -190 to -185°C. ETD was determined using the Laplace transform method and post-thaw viability was evaluated using a trypan blue exclusion assay. The extended Maxwell-Eucken equation estimated intracellular ETD, which decreased with increasing cooling rates as the tissues approached the vitrification state. There was also a correlation between increasing estimated ETD and decreasing viability. Therefore, estimated ETD can serve as the evaluation index when comparing the differing cryopreservation states of biological tissue.

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Format PDF

Pages : 21-28

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  • Prix public

    20 €

  • Prix membre*

    15 €

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Détails

  • Titre original : Effective thermal diffusivity as an index for evaluation of the state in cryopreserved artificial tissue.
  • Identifiant de la fiche : 2007-1314
  • Langues : Anglais
  • Source : Low Temperature Medicine - vol. 32 - n. 2
  • Date d'édition : 06/2006

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