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Effective thermal diffusivity as an index for evaluation of the state in cryopreserved artificial tissue.

Author(s) : OKANIWA K., UJIHIRA M., MATSUMURA Y., et al.

Type of article: Article

Summary

Estimated intracellular effective thermal diffusivity (ETD) was used to evaluate the viability of cryopreserved biological tissue. Artificial tissues were prepared using human fibroblasts cultured for 2 days in a collagen sponge. The artificial tissue was frozen at cooling rates of 30 to 700°C/min from 4 to -196°C in culture medium containing propylene glycol. The temperature of the artificial tissue was then increased stepwise from -190 to -185°C. ETD was determined using the Laplace transform method and post-thaw viability was evaluated using a trypan blue exclusion assay. The extended Maxwell-Eucken equation estimated intracellular ETD, which decreased with increasing cooling rates as the tissues approached the vitrification state. There was also a correlation between increasing estimated ETD and decreasing viability. Therefore, estimated ETD can serve as the evaluation index when comparing the differing cryopreservation states of biological tissue.

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Format PDF

Pages: 21-28

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Details

  • Original title: Effective thermal diffusivity as an index for evaluation of the state in cryopreserved artificial tissue.
  • Record ID : 2007-1314
  • Languages: English
  • Source: Low Temperature Medicine - vol. 32 - n. 2
  • Publication date: 2006/06

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