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Longévité des graines entreposées cryogéniquement.

Longevity of cryogenically stored seeds.

Auteurs : WALTERS C., WHEELER L., STANWOOD P. C.

Type d'article : Article

Résumé

Though cryogenic storage is presumed to provide nearly infinite longevity to cells, the actual shelf life achieved under ultra-cold temperatures has not been addressed theoretically or empirically. The authors report measurable changes in germination of dried seeds stored under liquid nitrogen conditions for more than 10 years. There was considerable variability in the extent of deterioration among species and accessions within a species. Aging time courses for lettuce seeds stored at temperatures between 50 and -196 °C were fit to a form of the Avrami equation to determine rate coefficients and predict half-life of accessions. A reduction in the temperature dependency on aging rate, determined as a break in the Arrhenius plot, occurred at about -15 °C, and this resulted in faster deterioration than anticipated from extrapolation of kinetics measured at higher temperatures. The break in Arrhenius behaviour occurred at temperatures in between the glass transition temperature (28 °C) and the Kauzmann temperature (-42 °C) and coincided with a major triacylglycerol phase change (-40 to -7 °C). In spite of the faster than anticipated deterioration, cryogenic storage clearly prolonged shelf life of lettuce seeds with half-lives projected as approximately 500 and 3400 years for fresh lettuce seeds stored in the vapour and liquid phases of liquid nitrogen, respectively. The benefit of low temperature storage (-18 or -135 °C) on seed longevity was progressively lost if seeds were first stored at 5 °C. These results demonstrate that lowering storage temperature progressively increases longevity of seeds. However, cryogenic temperatures were not sufficient to stop deterioration, especially if initial stages of aging were allowed to progress at higher storage temperatures.

Détails

  • Titre original : Longevity of cryogenically stored seeds.
  • Identifiant de la fiche : 2005-0442
  • Langues : Anglais
  • Source : Cryobiology - vol. 48 - n. 3
  • Date d'édition : 06/2004

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