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Cryobanque pour la cryoconservation des ressources génétiques végétales à I'Académie russe des sciences.

Cryobank of plant genetic resources in Russian Academy of Sciences.

Auteurs : POPOV A. S., POPOVA E. V., NIKISHINA T. V., et al.

Type d'article : Article, Article de la RIF

Résumé

Cryopreservation is the most reliable method for long-term storage of plant genetic resources. A review of cell injury by ice crystals and dehydration during a freeze-thaw cycle is given. For successful regeneration of plants and cultures after cryopreservation of their cells, the development of reliable cryopreservation procedure is required including preliminary cultivation, treatment by cryoprotectors, freezing by different methods, thawing and recultivation. Up to now, 27 cell lines successfully resumed their growth after storage in liquid nitrogen and preserved their specific features and biosynthetic potential. Besides, shoot tips of 40 cultivars of potato, rose, strawberry and raspberry regenerated plants both in vitro and in vivo after cryopreservation. The longest storage duration was 25 years. Today, 24 cell strains of rare medicinal plants, shoot tips of seven cultivars of strawberry and raspberry and seeds of 250 endangered plant species collected over all Russian territory are continuously stored in liquid nitrogen.

Documents disponibles

Format PDF

Pages : 403-410

Disponible

  • Prix public

    20 €

  • Prix membre*

    Gratuit

* meilleur tarif applicable selon le type d'adhésion (voir le détail des avantages des adhésions individuelles et collectives)

Détails

  • Titre original : Cryobank of plant genetic resources in Russian Academy of Sciences.
  • Identifiant de la fiche : 2006-1520
  • Langues : Anglais
  • Source : International Journal of Refrigeration - Revue Internationale du Froid - vol. 29 - n. 3
  • Date d'édition : 05/2006

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