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Mise en oeuvre de techniques de cryoconservation d'ail dans le dispositif national de germoplasme de plantes.

Implementation of garlic cryopreservation techniques in the national plant germplasm system.

Auteurs : ELLIS D., SKOGERBOE D., ANDRE C., et al.

Type d'article : Article

Résumé

The USDA-ARS National Plant Germplasm System (NPGS) maintains over 20 garlic (Allium sativum) accessions. The accessions are grown in the field each year. The cryopreservation methods involve the use of 15% v/v DMSO, 15% v/v ethylene glycol, 30% v/w glycerol, 0.4 M sucrose or 50% v/w sucrose, 50% v/w glycerol. Ten of twelve garlic accessions were successfully cryopreserved using either of these solutions. Five genotypes exhibited best regrowth after exposure to the second solution, and 3 genotypes performed equally well with either solution.

Détails

  • Titre original : Implementation of garlic cryopreservation techniques in the national plant germplasm system.
  • Identifiant de la fiche : 2006-2680
  • Langues : Anglais
  • Source : CryoLetters - vol. 27 - n. 2
  • Date d'édition : 03/2006

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