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Implementation of garlic cryopreservation techniques in the national plant germplasm system.

Author(s) : ELLIS D., SKOGERBOE D., ANDRE C., et al.

Type of article: Article

Summary

The USDA-ARS National Plant Germplasm System (NPGS) maintains over 20 garlic (Allium sativum) accessions. The accessions are grown in the field each year. The cryopreservation methods involve the use of 15% v/v DMSO, 15% v/v ethylene glycol, 30% v/w glycerol, 0.4 M sucrose or 50% v/w sucrose, 50% v/w glycerol. Ten of twelve garlic accessions were successfully cryopreserved using either of these solutions. Five genotypes exhibited best regrowth after exposure to the second solution, and 3 genotypes performed equally well with either solution.

Details

  • Original title: Implementation of garlic cryopreservation techniques in the national plant germplasm system.
  • Record ID : 2006-2680
  • Languages: English
  • Source: CryoLetters - vol. 27 - n. 2
  • Publication date: 2006/03

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